Human milk pasteurisation reduces pre-lipolysis but not digestive lipolysis and moderately decreases intestinal lipid uptake in a combination of preterm infant in vitro models - l'unam - université nantes angers le mans
Journal Articles Food Chemistry Year : 2020

Human milk pasteurisation reduces pre-lipolysis but not digestive lipolysis and moderately decreases intestinal lipid uptake in a combination of preterm infant in vitro models

Abstract

Donor human milk, pasteurised for safety reasons, is the first alternative for feeding preterm infants when mothers' own milk is unavailable. Breastmilk pasteurization impact on lipid digestion and absorption was evaluated by a static in vitro digestion model for preterm infants coupled with intestinal absorption using Caco-2/TC7 cell. Lipid absorption was quantified by digital image analysis of lipid droplets, by measurement of triglyceride concentration in basolateral medium and by analysing the expression of major genes involved. After in vitro digestion, lipolysis extent was 13% lower in pasteurised human milk (PHM) than in raw human milk (RHM). In Caco-2/TC7 cells, the number of lipid droplets was identical for both milk types, while the mean droplet area was 17% smaller with PHM. Altogether, pasteurisation decreased the pre-lipolysis of human milk. This initial difference in free fatty acid amount was only partially buffered by the subsequent processes of in vitro digestion and cellular lipid absorption.
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Dates and versions

hal-04779516 , version 1 (13-11-2024)

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Marine Vincent, Olivia Ménard, Julie Etienne, Jordane Ossemond, Annie Durand, et al.. Human milk pasteurisation reduces pre-lipolysis but not digestive lipolysis and moderately decreases intestinal lipid uptake in a combination of preterm infant in vitro models. Food Chemistry, 2020, 329, pp.126927. ⟨10.1016/j.foodchem.2020.126927⟩. ⟨hal-04779516⟩
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