<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02977712</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-14T22:31:12+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Differential effects of Bcl-2 and caspases on mitochondrial permeabilization during endogenous or exogenous reactive oxygen species-induced cell death</title>
            <title xml:lang="en" type="sub">A comparative study of H 2 O 2 , paraquat, t-BHP, etoposide and TNF-α-induced cell death</title>
            <author role="aut">
              <persName>
                <forename type="first">Vincent</forename>
                <surname>Rincheval</surname>
              </persName>
              <email type="md5">2895df08ad802a79ad7953742796298a</email>
              <email type="domain">uvsq.fr</email>
              <idno type="idhal" notation="numeric">1080043</idno>
              <idno type="halauthorid" notation="string">168937-1080043</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie</forename>
                <surname>Bergeaud</surname>
              </persName>
              <idno type="halauthorid">1420029-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Lise</forename>
                <surname>Mathieu</surname>
              </persName>
              <idno type="halauthorid">1420030-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jacqueline</forename>
                <surname>Leroy</surname>
              </persName>
              <idno type="halauthorid">200604-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Arnaud</forename>
                <surname>Guillaume</surname>
              </persName>
              <idno type="halauthorid">1420031-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Bernard</forename>
                <surname>Mignotte</surname>
              </persName>
              <email type="md5">60683a336031f028bb1e21c64ca5576e</email>
              <email type="domain">uvsq.fr</email>
              <idno type="idhal" notation="string">bernard-mignotte</idno>
              <idno type="idhal" notation="numeric">7489</idno>
              <idno type="halauthorid" notation="string">5152-7489</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/A-3499-2009</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-8512-8518</idno>
              <idno type="IDREF">https://www.idref.fr/032984677</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-3499-2009</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">N</forename>
                <surname>Le Floch</surname>
              </persName>
              <idno type="halauthorid">2052179-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">J.-L</forename>
                <surname>Vayssière</surname>
              </persName>
              <idno type="halauthorid">2052180-0</idno>
              <affiliation ref="#struct-20765"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Bernard</forename>
                <surname>Mignotte</surname>
              </persName>
              <email type="md5">60683a336031f028bb1e21c64ca5576e</email>
              <email type="domain">uvsq.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2020-10-26 17:48:45</date>
              <date type="whenModified">2025-07-16 10:44:02</date>
              <date type="whenReleased">2020-10-26 17:55:30</date>
              <date type="whenProduced">2012</date>
              <date type="whenEndEmbargoed">2020-10-26</date>
              <ref type="file" target="https://uvsq.hal.science/hal-02977712v1/document">
                <date notBefore="2020-10-26"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://uvsq.hal.science/hal-02977712v1/file/CBTO-S-11-00220-soumis%20%281%29.pdf" id="file-2977712-2624816">
                <date notBefore="2020-10-26"/>
              </ref>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/VQC-JQ1R9H8R-V/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="333803">
                <persName>
                  <forename>Bernard</forename>
                  <surname>Mignotte</surname>
                </persName>
                <email type="md5">60683a336031f028bb1e21c64ca5576e</email>
                <email type="domain">uvsq.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02977712</idno>
            <idno type="halUri">https://uvsq.hal.science/hal-02977712</idno>
            <idno type="halBibtex">rincheval:hal-02977712</idno>
            <idno type="halRefHtml">&lt;i&gt;Cell Biology and Toxicology&lt;/i&gt;, 2012, 28, pp.239-253. &lt;a target="_blank" href="https://dx.doi.org/10.1007/s10565-012-9219-9"&gt;&amp;#x27E8;10.1007/s10565-012-9219-9&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Cell Biology and Toxicology, 2012, 28, pp.239-253. &amp;#x27E8;10.1007/s10565-012-9219-9&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-2977712-2624816"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="EPHE">École Pratique des Hautes Études</idno>
            <idno type="stamp" n="UVSQ">Université de Versailles Saint-Quentin-en-Yvelines</idno>
            <idno type="stamp" n="LGBC">Laboratoire de génétique et biologie cellulaire</idno>
            <idno type="stamp" n="PSL">Université Paris sciences et lettres</idno>
            <idno type="stamp" n="TEST-DEV">TEST-DEV</idno>
            <idno type="stamp" n="EPHE-PSL" corresp="PSL">École Pratique des Hautes Études - PSL</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Differential effects of Bcl-2 and caspases on mitochondrial permeabilization during endogenous or exogenous reactive oxygen species-induced cell death</title>
                <title xml:lang="en" type="sub">A comparative study of H 2 O 2 , paraquat, t-BHP, etoposide and TNF-α-induced cell death</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Vincent</forename>
                    <surname>Rincheval</surname>
                  </persName>
                  <email type="md5">2895df08ad802a79ad7953742796298a</email>
                  <email type="domain">uvsq.fr</email>
                  <idno type="idhal" notation="numeric">1080043</idno>
                  <idno type="halauthorid" notation="string">168937-1080043</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie</forename>
                    <surname>Bergeaud</surname>
                  </persName>
                  <idno type="halauthorid">1420029-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Lise</forename>
                    <surname>Mathieu</surname>
                  </persName>
                  <idno type="halauthorid">1420030-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jacqueline</forename>
                    <surname>Leroy</surname>
                  </persName>
                  <idno type="halauthorid">200604-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Arnaud</forename>
                    <surname>Guillaume</surname>
                  </persName>
                  <idno type="halauthorid">1420031-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Bernard</forename>
                    <surname>Mignotte</surname>
                  </persName>
                  <email type="md5">60683a336031f028bb1e21c64ca5576e</email>
                  <email type="domain">uvsq.fr</email>
                  <idno type="idhal" notation="string">bernard-mignotte</idno>
                  <idno type="idhal" notation="numeric">7489</idno>
                  <idno type="halauthorid" notation="string">5152-7489</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/A-3499-2009</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-8512-8518</idno>
                  <idno type="IDREF">https://www.idref.fr/032984677</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/A-3499-2009</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">N</forename>
                    <surname>Le Floch</surname>
                  </persName>
                  <idno type="halauthorid">2052179-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">J.-L</forename>
                    <surname>Vayssière</surname>
                  </persName>
                  <idno type="halauthorid">2052180-0</idno>
                  <affiliation ref="#struct-20765"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11552</idno>
                <idno type="issn">0742-2091</idno>
                <idno type="eissn">1573-6822</idno>
                <title level="j">Cell Biology and Toxicology</title>
                <imprint>
                  <publisher>Springer Verlag</publisher>
                  <biblScope unit="volume">28</biblScope>
                  <biblScope unit="pp">239-253</biblScope>
                  <date type="datePub">2012</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/s10565-012-9219-9</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">cell death</term>
                <term xml:lang="en">Mitochondria</term>
                <term xml:lang="en">Bcl-2</term>
                <term xml:lang="en">caspases</term>
                <term xml:lang="en">antioxidants.</term>
                <term xml:lang="en">Reactive oxygen species</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bc.bc">Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>In this study, we have compared several features of cell death triggered by classical inducers of apoptotic pathways (etoposide and tumour necrosis factor (TNF)-α) versus exogenous reactive oxygen species (ROS; hydrogen peroxide (H 2 O 2), tert-butyl hydroper-oxide (t-BHP)) or a ROS generator (paraquat). Our aim was to characterize relationships that exist between ROS, mitochondrial perturbations, Bcl-2 and caspases, depending on source and identity of ROS. First, we have found that these five inducers trigger oxidative stress, mitochondrial membrane permeabilization (MMP), cy-tochrome c (cyt c) release from mitochondria and cell death. In each case, cell death could be inhibited by several antioxidants, showing that it is primarily ROS dependent. Second, we have highlighted that during etoposide or TNF-α treatments, intracellular ROS level, MMP and cell death are all regulated by caspases and Bcl-2, with caspases acting early in the process. Third, we have demonstrated that H 2 O 2-induced cell death shares many of these characteristics with etoposide and TNF-α, whereas t-BHP induces both caspase-dependent and caspase-independent cell death. Surprisingly , paraquat-induced cell death, which harbours some characteristics of apoptosis such as cyt c release and caspase-3 activation, is not modulated by Bcl-2 and caspase inhibitors, suggesting that paraquat also triggers non-apoptotic cell death signals. On the one hand, these results show that endogenous or exogenous ROS can trigger multiple cell death pathways with Bcl-2 and caspases acting differentially. On the other hand, they suggest that H 2 O 2 could be an important mediator of etoposide and TNF-α-dependent cell death since these inducers trigger similar phenotypes.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-20765" status="OLD">
          <idno type="IdRef">184505682</idno>
          <idno type="ISNI">0000 0004 0371 1043</idno>
          <idno type="RNSR">201019677Z</idno>
          <idno type="ROR">https://ror.org/00edzht24</idno>
          <orgName>Laboratoire de génétique et biologie cellulaire</orgName>
          <orgName type="acronym">LGBC</orgName>
          <date type="end">2023-03-29</date>
          <desc>
            <address>
              <addrLine>Universite de VersaillesSaint-Quentin-en-Yvelines UFR de la Santé Simone Veil 2 avenue de la source de la Bièvre78180 Montigny le Bretonneux</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.lgbc.uvsq.fr/</ref>
          </desc>
          <listRelation>
            <relation name=" UR4589" active="#struct-81173" type="direct"/>
            <relation active="#struct-110691" type="direct"/>
            <relation active="#struct-564132" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-81173" status="VALID">
          <idno type="IdRef">03082057X</idno>
          <idno type="ISNI">0000 0001 2323 0229 </idno>
          <idno type="ROR">https://ror.org/03mkjjy25</idno>
          <orgName>Université de Versailles Saint-Quentin-en-Yvelines</orgName>
          <orgName type="acronym">UVSQ</orgName>
          <date type="start">1991-07-22</date>
          <desc>
            <address>
              <addrLine>55 avenue de Paris - 78035 Versailles cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.uvsq.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-110691" status="VALID">
          <idno type="IdRef">026375478</idno>
          <idno type="ISNI">0000000121955365</idno>
          <idno type="ROR">https://ror.org/046b3cj80</idno>
          <orgName>École Pratique des Hautes Études</orgName>
          <orgName type="acronym">EPHE</orgName>
          <date type="start">1868-01-01</date>
          <desc>
            <address>
              <addrLine>4-14 Rue Ferrus, 75014 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ephe.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-564132" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-564132" status="VALID">
          <idno type="IdRef">241597595</idno>
          <idno type="ISNI">0000 0004 1784 3645</idno>
          <idno type="ROR">https://ror.org/013cjyk83</idno>
          <orgName>Université Paris Sciences et Lettres</orgName>
          <orgName type="acronym">PSL</orgName>
          <desc>
            <address>
              <addrLine>60 rue Mazarine 75006 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.psl.eu/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>