Screening antivirals with a mCherry-expressing recombinant bovine respiratory syncytial virus: a proof of concept using cyclopamine
Résumé
Abstract Bovine respiratory syncytial virus (BRSV) is a pathogenic pneumovirus and a major cause of acute respiratory infections in calves. Although different vaccines are available against BRSV, their efficiency remains limited, and no efficient and large-scale treatment exists. Here, we developed a new reverse genetics system for BRSV expressing the red fluorescent protein mCherry, based on a field strain isolated from a sick calf in Sweden. Although this recombinant fluorescent virus replicated slightly less efficiently compared to the wild type virus, both viruses were shown to be sensitive to the natural steroidal alkaloid cyclopamine, which was previously shown to inhibit human RSV replication. Our data thus point to the potential of this recombinant fluorescent BRSV as a powerful tool in preclinical drug discovery to enable high throughput compound screening.
Mots clés
Bovine respiratory syncytial virus, mCherry reverse genetics, antiviral drugs, cyclopamine
Bovine respiratory syncytial virus mCherry reverse genetics antiviral drugs cyclopamine Handling editor: Vincent Béringue
Bovine respiratory syncytial virus
mCherry reverse genetics
antiviral drugs
cyclopamine Handling editor: Vincent Béringue
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